• Health and Nutrition

Nuclear Receptors, Metabolic and Cardiovascular Diseases (RNMMC)

Research unit - U 1011

Description

Our laboratory studies the biological and molecular mechanisms controlling the development and progression of type 2 diabetes and its cardiovascular complications, in order to develop preventive and therapeutic strategies. We study the regulation of genes involved in these pathologies and the consequences of their deregulation, with a particular interest in specific transcription factors: nuclear receptors which constitute potential therapeutic targets. Our previous studies have identified nuclear receptors, such as PPARs and LXRs, as metabolic regulators and pharmacological targets, and have led to a better understanding of the side effects of synthetic ligands of nuclear receptors currently used in therapies or under development. We are open to all academic and industrial collaborations and propose services in line with our expertise.

Contacts

  • Bart Staels
    Director

Informations

1 rue du Professeur Calmette
Institut Pasteur de Lille, BP 245
59019 LILLE

https://u1011.pasteur-lille.fr/accueil/

Effectif

Effectif total : 129

Personnel de recherche : 53

Personnel d'appui à la recherche : 56

Expertises

Skills

• Implication of nuclear receptors in perturbations of metabolism and energy homeostasis
• Hallmark of Type 2 Diabetes
• Regulatory role of nuclear receptors in the inflammatory process in adipose tissue, the liver and vascular wall
• Molecular mechanisms by which nuclear receptors may modulate the progression to Type 2 Diabetes
• Pathophysiology of early (metabolic syndrome) and late stages of Type 2 Diabetes, obesity and cardiovascular diseases
• Contribution of nuclear receptors to the pathophysiology of Type 2 Diabetes and cardiovascular diseases
• Metabolic regulators (FXR, Reverba, RORa and orphan receptors)
• Therapeutic potential of selective ligands

Example(s) of projects

• ERC Immunobile - Bile acid, immunometabolism, lipid and glucose homeostasis
• ANR-NASHILCCD8 – Contribution of ILCs and CD8 T cells to non-alcoholic fatty liver disease and its progression to hepatocarcinoma (2019-2022)
• ANR-CALMOS – Mitochondrial calcium homeostasis and cardiac arrhytmias in the metabolic syndrome (2019-2022)
• ANR-MET Intestin – Acute effect of metformin on intestinal sodium-glucose co-transport (2019-2022)

Example(s) of publications

• Human aortic valve interstitial cells display proangiogenic properties during calcific aortic valve disease. N. Gendron, M. Rosa, A. Blandinières, Y. Sottejeau, E. Rossi, E. Van Belle, S. Idelcadi, S. Lecourt, A. Vincentelli, A. Cras, R. Jashari, R. Chocron, Y. Baudouin, T. Pamart, I. Bieche, N. Nevo, B. Cholley, J. Rancic, B. Staels, P. Gaussem, A. Dupont, A. Carpentier, S. Susen, D.M. Smadja. Arterioscler. Thromb. Vasc. Biol., 2021, 41, 415-429.
• Beyond the rule of 5: impact of PEGylation with various polymer sizes on pharmacokinetic properties, structure-properties relationships of mPEGylated small agonists of TGR5 receptor. V. Hoguet, M. Lasalle, M. Maingot, G. Dequirez, R. Boulahjar, F. Leroux, C. Piveteau, A. Herledan, A. Biela, J. Dumont, O. Chavez-Talavera, L. Belloy, I. Duplan, N. Hennuter, L. Butruille, S. Lestavel, E. Sevin, M. Culot, F. Gosselet, B. Staels, B. Deprez, A. Tailleux, J. Charton. J. Med. Chem., 2021, 64, 1593-1610.
• Cholangiopathy and biliary fibrosis in Cyp2c70-deficient mice are fully reversed by ursodeoxycholic acid. J.F. De Boer, H.D. De Vries, A. Palmiotti, R. Li, M. Doestzada, J.A. Hoogerland, J. Fu, A.M. La Rose, M. Westerterp, N.L. Mulder, M.V. Hovingh, M. Koehorst, N.J. Kloosterhuis, J.C. Wolters, V.W. Bloks, J.T. Haas, D. Dombrowicz, B. Staels, B. Van De Sluis, F. Kuipers. Cell. Mol. Gastroenterol. Hepatol., 2021, 11, 1045-1069.
• Apolipoprotein A5 controls fructose-induced metabolic dysregulation in mice. C. Ress, J. Dobner, K. Rufinatscha, B. Staels, M. Hofer, S. Folie, B. Radlinger, T.E. Adolph, E.M. Rubin, M. Roden, H. Tilg, S. Kaser. Nutr. Metab. Cardiovasc. Dis., 2021, 31, 972-978.
• NASH-related increases in plasma bile acid levels depend on insulin resistance. G. Grzych, O. Chavez-Talavera, A. Descat, D. Thuillier, A. Verrijken, M. Kouach, V. Legry, H. Verkindt, V. Raverdy, B. Legendre, R. Caiazzo, L. Van Gaal, J.F. Goossens, R. Paumelle, S. Francque, F. Pattou, J.T. Haas, A. Tailleux, B. Staels. JHEP Reports, 2021, 3, 100222.

Discover the full list of publications here.

Collaborations/Partners/Scientific clients

National:
Pasteur Institute of Paris, University of Paris, Sorbonne University, University of Montpellier

International:
University of Antwerp (Belgium), University of Liege (Belgium), Maastricht University (Germany)

Collaborations/Partners/Private Clients

The unit collaborates or has collaborated with many companies (Genfit, Servier, Tiger, Sanofi, Oseo-Anvar, Galderma, Pfizer, LFB, Corwave, Carmat, Stago, Sebia, Siemens,…)

Services offers

Applications sectors

  • Health / wellness
  • Education / Training
  • Science / Research

Services provided

Biochemistry
• Measurement of circulating biochemical parameters (glucose, insulin, transaminases, free fatty acids, etc.)
• Measurement of lipid and lipoprotein concentrations
• Determination of lipid distribution profiles
• Determination of bile acid profiles (21 species) in biological environments (plasma, feces, bile)

Histology (Ultra-microtomy, Immunohistochemistry, Microscopy, Laser capture microdissection)

Immunophenotyping (Flow cytometry)

Cellular Biology
• Cell lines (Human and murine hepatic cells, murine preadipocyte cells, murine fibroblastic cells, human leukocyte cells, murine beta-pancreatic cells, human kydney cells, murine enteroendocrine cells…)
• Primary cell cultures (Murine and human hepatocytes, murine and human monocytes and macrophages)
• Human and murine intestinal organoids

Molecular Biology (Microarray, Proteomic analysis, Real time PCR)

In Vivo
• Animal imaging (scanner/MRI small animal)
• Bone marrow transplantation
• Measures of animal metabolic function
• Plethysmography (lung function)
• Measures of animal cardiovascular function
• Genetically modified animal models
• Allergy models

Training offers

Master Bioloy-Health

Consulting services

We provide consulting services related to all our areas of expertise.

Equipments

We possess a wide range of equipment regrouped within 6 platforms:
• Biochemistry platform (Konelab 20 system to measure classical biochemical parameters (glucose, lipids, transaminases...), HPLC, ultracentrifuge
• Immunophenotyping platform (FORTESSA X20 analyzer, cell sorter INFLUX, mass cytometry system CyTOF 2)
• Histology platform (device for tissue preparation STP 120 Spin, paraffin embedding device Leica EG1160, microtome Leica RM2145, cryostats Leica CM3050S and Microm HM560, staining devices Leica Autostainer XL and Lab Vision 360-2D, microscopes Leitz DMRB, Leica MZ6 et Nikon Ti, microdissector Arcturus XT, bioanalyzer Agilent 2100)
• Epigenetics platform (Affymetrix GeneChip(R) device)
• Animal phenotyping platform (system for measuring exercise endurance, metabolic cages)
• Oxygraphy platform (oxygraph OROBOROS)

Biological materials

Cell lines
Human and murine hepatic cells, murine preadipocyte cells, murine fibroblastic cells, human leukocyte cells, murine beta-pancreatic cells, human kydney cells, murine enteroendocrine cells…

Primary cell cultures
Murine and human hepatocytes, murine and human monocytes and macrophages

Human and murine intestinal organoids

Ecosystem

Affiliated institutions / organisations

Inserm
University of Lille
Institut Pasteur de Lille

Partner institution(s)

CHU de Lille

Equipex/EquipEx+/ESR

Doctoral schools

Competitive cluster

Regional strategic areas of activity

  • Health and Nutrition
    • New therapeutic approaches

Request for modification

You enter the « edit request » mode. After sending your modifications, they will first be validated by the Plug in labs Ouest team, before appearing on the platform.

Thematics

Current thematics

  • Health and Nutrition
    • New therapeutic approaches
Title

Current content

Nuclear Receptors, Metabolic and Cardiovascular Diseases
Type

Current content

Research unit
Description

Current content

Our laboratory studies the biological and molecular mechanisms controlling the development and progression of type 2 diabetes and its cardiovascular complications, in order to develop preventive and therapeutic strategies. We study the regulation of genes involved in these pathologies and the consequences of their deregulation, with a particular interest in specific transcription factors: nuclear receptors which constitute potential therapeutic targets. Our previous studies have identified nuclear receptors, such as PPARs and LXRs, as metabolic regulators and pharmacological targets, and have led to a better understanding of the side effects of synthetic ligands of nuclear receptors currently used in therapies or under development. We are open to all academic and industrial collaborations and propose services in line with our expertise.
Contact informations
Contact

Current content

Contact name Email phone number
STAELS Bart bart.staels@pasteur-lille.fr (+33) 3 20 96 40 40
URL

Current content

https://u1011.pasteur-lille.fr/accueil/

Address

Current content

1 rue du Professeur Calmette
Institut Pasteur de Lille, BP 245
59019 LILLE
Keywords

Current content

Nuclear receptors, Diabetes, Metabolic diseases, Cardiovascular diseases, Obesity, Liver diseases
Our search domains
Skills

Current content

• Implication of nuclear receptors in perturbations of metabolism and energy homeostasis • Hallmark of Type 2 Diabetes • Regulatory role of nuclear receptors in the inflammatory process in adipose tissue, the liver and vascular wall • Molecular mechanisms by which nuclear receptors may modulate the progression to Type 2 Diabetes • Pathophysiology of early (metabolic syndrome) and late stages of Type 2 Diabetes, obesity and cardiovascular diseases • Contribution of nuclear receptors to the pathophysiology of Type 2 Diabetes and cardiovascular diseases • Metabolic regulators (FXR, Reverba, RORa and orphan receptors) • Therapeutic potential of selective ligands
Workforce

Current content

Nombre de chercheurs : 53
Nombre de doctorants :
Nombre de personnel d'appui à la recherche : 56
Effectif total : 129
Example(s) of projects

Current content

• ERC Immunobile - Bile acid, immunometabolism, lipid and glucose homeostasis • ANR-NASHILCCD8 – Contribution of ILCs and CD8 T cells to non-alcoholic fatty liver disease and its progression to hepatocarcinoma (2019-2022) • ANR-CALMOS – Mitochondrial calcium homeostasis and cardiac arrhytmias in the metabolic syndrome (2019-2022) • ANR-MET Intestin – Acute effect of metformin on intestinal sodium-glucose co-transport (2019-2022)
Example(s) of publications

Current content

• Human aortic valve interstitial cells display proangiogenic properties during calcific aortic valve disease. N. Gendron, M. Rosa, A. Blandinières, Y. Sottejeau, E. Rossi, E. Van Belle, S. Idelcadi, S. Lecourt, A. Vincentelli, A. Cras, R. Jashari, R. Chocron, Y. Baudouin, T. Pamart, I. Bieche, N. Nevo, B. Cholley, J. Rancic, B. Staels, P. Gaussem, A. Dupont, A. Carpentier, S. Susen, D.M. Smadja. Arterioscler. Thromb. Vasc. Biol., 2021, 41, 415-429. • Beyond the rule of 5: impact of PEGylation with various polymer sizes on pharmacokinetic properties, structure-properties relationships of mPEGylated small agonists of TGR5 receptor. V. Hoguet, M. Lasalle, M. Maingot, G. Dequirez, R. Boulahjar, F. Leroux, C. Piveteau, A. Herledan, A. Biela, J. Dumont, O. Chavez-Talavera, L. Belloy, I. Duplan, N. Hennuter, L. Butruille, S. Lestavel, E. Sevin, M. Culot, F. Gosselet, B. Staels, B. Deprez, A. Tailleux, J. Charton. J. Med. Chem., 2021, 64, 1593-1610. • Cholangiopathy and biliary fibrosis in Cyp2c70-deficient mice are fully reversed by ursodeoxycholic acid. J.F. De Boer, H.D. De Vries, A. Palmiotti, R. Li, M. Doestzada, J.A. Hoogerland, J. Fu, A.M. La Rose, M. Westerterp, N.L. Mulder, M.V. Hovingh, M. Koehorst, N.J. Kloosterhuis, J.C. Wolters, V.W. Bloks, J.T. Haas, D. Dombrowicz, B. Staels, B. Van De Sluis, F. Kuipers. Cell. Mol. Gastroenterol. Hepatol., 2021, 11, 1045-1069. • Apolipoprotein A5 controls fructose-induced metabolic dysregulation in mice. C. Ress, J. Dobner, K. Rufinatscha, B. Staels, M. Hofer, S. Folie, B. Radlinger, T.E. Adolph, E.M. Rubin, M. Roden, H. Tilg, S. Kaser. Nutr. Metab. Cardiovasc. Dis., 2021, 31, 972-978. • NASH-related increases in plasma bile acid levels depend on insulin resistance. G. Grzych, O. Chavez-Talavera, A. Descat, D. Thuillier, A. Verrijken, M. Kouach, V. Legry, H. Verkindt, V. Raverdy, B. Legendre, R. Caiazzo, L. Van Gaal, J.F. Goossens, R. Paumelle, S. Francque, F. Pattou, J.T. Haas, A. Tailleux, B. Staels. JHEP Reports, 2021, 3, 100222. Discover the full list of publications here.
Collaborations/Partners/Scientific clients

Current content

National: Pasteur Institute of Paris, University of Paris, Sorbonne University, University of Montpellier International: University of Antwerp (Belgium), University of Liege (Belgium), Maastricht University (Germany)
Collaborations/Partners/Private Clients

Current content

The unit collaborates or has collaborated with many companies (Genfit, Servier, Tiger, Sanofi, Oseo-Anvar, Galderma, Pfizer, LFB, Corwave, Carmat, Stago, Sebia, Siemens,…)
Services offers
Training offers

Current content

Master Bioloy-Health
Services offers

Current content

Biochemistry • Measurement of circulating biochemical parameters (glucose, insulin, transaminases, free fatty acids, etc.) • Measurement of lipid and lipoprotein concentrations • Determination of lipid distribution profiles • Determination of bile acid profiles (21 species) in biological environments (plasma, feces, bile) Histology (Ultra-microtomy, Immunohistochemistry, Microscopy, Laser capture microdissection) Immunophenotyping (Flow cytometry) Cellular Biology • Cell lines (Human and murine hepatic cells, murine preadipocyte cells, murine fibroblastic cells, human leukocyte cells, murine beta-pancreatic cells, human kydney cells, murine enteroendocrine cells…) • Primary cell cultures (Murine and human hepatocytes, murine and human monocytes and macrophages) • Human and murine intestinal organoids Molecular Biology (Microarray, Proteomic analysis, Real time PCR) In Vivo • Animal imaging (scanner/MRI small animal) • Bone marrow transplantation • Measures of animal metabolic function • Plethysmography (lung function) • Measures of animal cardiovascular function • Genetically modified animal models • Allergy models
Consulting services

Current content

We provide consulting services related to all our areas of expertise.
Equipments
Equipements spécifiques

Current content

  • Analyzer of biochemical parameters
    Konelab 20/ Thermo Scientific
  • Flow cytometry analyzer
    FORTESSA X20/ BD Biosciences
  • Cell sorter
    INFLUX/ BD Biosciences
  • Mass cytometry system
    CyTOF 2/ Fluidigm
  • Bioanalyzer
    Agilent 2100/ Agilent
  • Microdissection
    Arcturus XT/ ThermoFisher
  • Oxygraph
    O2k/ Oroboros
  • Automated coloring machine
    Lab Vision 360-2D/ Thermo Scientific
Ecosystem
Affiliated institutions / organisations

Current content

  • Inserm
  • University of Lille
  • Institut Pasteur de Lille
Établissement(s) partenaire(s)

Current content

CHU de Lille
Unit(s) of attachment

Current content

Groups/Networks/Federations

Current content

Doctoral schools

Current content

Equipex/EquipEx+/ESR

Current content

Competitive cluster

Current content

Regional strategic areas of activity

Current content

  • Health and Nutrition
  • New therapeutic approaches
Personal informations
Back to top