• Health and Nutrition

Laboratory of Cell Physiology (PhyCell)

Research unit - U 1003


The scientific project of our laboratory encompasses fundamental aspects of ion channels’ biology with a particular focus on the consequences of their dysregulation during the development of prostate, pancreatic and colon cancers. Our experimental approaches include electrophysiology, imaging, biochemistry, molecular biology and flow cytometry techniques, validated in novel in vivo models. Our understanding of ion channels and some associated inflammasomes in pathological conditions allows us to develop biomarkers and innovative therapeutic approaches for a better management of "oncochannelopathies". Given the molecular complexity, tumor heterogeneity as well as resistance to anti-cancer therapies, we propose a strategy focused on ion channels and associated inflammasomes. This strategy has three components: (1) Identify a molecular "signature" that can be used for the development of a diagnostic/prognostic test for efficacy/toxicity to locoregional therapies in close collaboration with clinicians. (2) Develop the visualization and measurement of dynamic alterations of channels and inflammasomes during carcinogenesis. (3) Improve the current personalized medicine strategy against prostate, pancreatic and colorectal cancers.


  • Sandy Despeghel
  • Natacha Prevarskaya


Bâtiment SN3, Avenue Paul Langevin
Campus Cité Scientifique, Université de Lille


Laboratory of Cell Physiology


Effectif total : 47

Personnel de recherche : 22

Personnel d'appui à la recherche : 8



• Ion channels
• Single channels
• Ion channel pharmacology
• Intracellular signaling
• Calcium signaling
• Prostate cancer
• Pancreatic cancer
• Resistance to chemotherapy

Example(s) of projects

• Role of ion channels in tumor initiation and progression.
• TRPV6 regulation and targeting in cancer.
• Calcium-permeable channels in cancer-related senescence.

Example(s) of publications

• TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1. Genova T, Grolez GP, Camillo C, Bernardini M, Bokhobza A, Richard E, Scianna M, Lemonnier L, Valdembri D, Munaron L, Philips MR, Mattot V, Serini G, Prevarskaya N, Gkika D, Pla AF. J Cell Biol. 2017 Jul 3;216(7):2107-2130. doi: 10.1083/jcb.201506024. 🡭
• The calcium-cancer signalling nexus. Monteith GR, Prevarskaya N, Roberts-Thomson SJ. Nat Rev Cancer. 2017 Jun;17(6):367-380. doi: 10.1038/nrc.2017.18. 🡭
• Epidermal TRPM8 channel isoform controls the balance between keratinocyte proliferation and differentiation in a cold-dependent manner. Bidaux G, Borowiec AS, Gordienko D, Beck B, Shapovalov GG, Lemonnier L, Flourakis M, Vandenberghe M, Slomianny C, Dewailly E, Delcourt P, Desruelles E, Ritaine A, Polakowska R, Lesage J, Chami M, Skryma R, Prevarskaya N. Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3345-54. doi: 10.1073/pnas.1423357112. 🡭
• TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity. Gkika D, Lemonnier L, Shapovalov G, Gordienko D, Poux C, Bernardini M, Bokhobza A, Bidaux G, Degerny C, Verreman K, Guarmit B, Benahmed M, de Launoit Y, Bindels RJ, Fiorio Pla A, Prevarskaya N. J Cell Biol. 2015 Jan 5;208(1):89-107. doi: 10.1083/jcb.201402076. 🡭
• Remodeling of channel-forming ORAI proteins determines an oncogenic switch in prostate cancer. Dubois C, Vanden Abeele F, Lehen'kyi V, Gkika D, Guarmit B, Lepage G, Slomianny C, Borowiec AS, Bidaux G, Benahmed M, Shuba Y, Prevarskaya N. Cancer Cell. 2014 Jul 14;26(1):19-32. doi: 10.1016/j.ccr.2014.04.025. 🡭

Collaborations/Partners/Scientific clients

University de Valenciennes et du Hainaut-Cambresis, Institut Pasteur Lille, Centrale Lille, INSA Lyon, University Claude Bernard Lyon, CHU Lille, University of Paris, University of Tours, University of Grenoble Alpes, University of Clermont Auvergne
Institute of Physiology Bogomoletz (Ukraine), University of Turin (Italy), University of Münster (Germany)

Services offers

Applications sectors

  • Health / wellness
  • Science / Research

Services provided

• Electrophysiological study of ion channels (whole cell, single channels...)
• Screening of pharmacological molecules and antibodies modulating ion channels
• Study of signaling pathways
• Calcium imaging

Consulting services

We provide consulting services related to all our areas of expertise.


Our laboratory is equipped with:
- 5 patch-clamp stations
- a "Port-A-Patch" system (Nanion)
- two calcium imaging stations
- 2 confocal microscopes
- as well as basic laboratory equipment (cell culture equipment, cell and molecular biology, etc.).


Affiliated institutions / organisations

Partner institution(s)



Doctoral schools

Regional strategic areas of activity

  • Health and Nutrition
    • Food ingredients
    • New therapeutic approaches