• Health and Nutrition

Mass spectrometry for Omics



Our platform is specialized in proteomics and mass spectrometry for biological and clinical research applications. In particular, our platform offers a wide range of analyses and a high level of expertise in the fields of microproteomics, molecular imaging by mass spectrometry, the study of protein-protein interactions (PPI) and in vivo mass spectrometry. We have extensive experience and state-of-the-art instrumentation for sample preparation, imaging analysis by mass spectrometry or spatial proteomics and data analysis. We are open to academic, clinical and industrial collaborations, and propose both methodological / technological development and services.


  • Michel Salzet
    Scientific manager
  • Fabrice Bray
  • Isabelle Fournier
    Scientific manager


Bât SN3 et Bât C4
Campus Cité Scientifique, Université de Lille


Chiffres clés

Number of regional projects per year = 19
Number of national projects per year = 8
Number of international projects per year = 8
Number of industrial projects = 11
Participation in European projects = 3
Number of analyses per year : 6000
Stored data = 200 TB


Effectif total : 10



• Proteomics
• Spatial Proteomics
• Microproteomics
• Omics
• Mass Spectrometry
• Molecular Imaging by Mass Spectrometry
• In vivo mass spectrometry
• Protein analysis
• Protein-protein interactions

Example(s) of projects

• Study of inflammation processes related to acne and atopic dermatitis - proteomic analyses of sussion bubble roofs were performed on a cohort of 16 patients (8 with atopic dermatitis).
• Search for diagnostic and prognostic markers of pancreatic cancer in mouse models - mass spectrometry mapping of neoplastic lesions (MALDI-MSI) combined with microproteomics to identify new progression markers.
• Comparative proteomic and transcriptomic analysis of follistatin-induced skeletal muscle hypertrophy
• Analysis of the local microbiota in breast cancer - localized microproteomic analysis of 12 patients
• Proteomic study of the effect of cell passage number and stimulation conditions in primary human fibroblasts

Collaborations/Partners/Scientific clients

University of Lyon, Univ. of Nice, Institute of Regenerative Medicine and Biotherapies, Montpellier, University of Rouen, University of the Littoral, University of Lorraine, University of Southern Brittany, Centre for Research and Restoration of the Museums of France.

University of Montreal, Canada; Academy of Sciences Bratislava, Slovakia; Università del Salento, Lecce, Italy; Univ. Mons, Belgium;
University of Florida, USA; University of Ioannina, Greece; Luxembourg Institute of Health, Luxembourg; University of Twente, Netherlands; Gent University, Belgium

Collaborations/Partners/Private Clients

Mäder, Lesaffre, Roquette, Bifinove, Laboratoire Nicolas Garnier, Imabiotech, LCH, Macopharma, OCR, Pierre Fabre, Servier, L’Oréal, GENFIT

Services offers

Applications sectors

  • Health / wellness
  • Agri-food industry
  • Art / Culture
  • Cosmetics
  • Science / Research

Services provided

• Preparation of biological and clinical samples (extraction, fractionation...)
• Separation (1D, 2D, Gel Free, 2D-DIGE, various chromatographies)
• Identification and relative quantification of proteins (exact mass, HR-MS coupling) by different non-targeted approaches (DIA, DDA) with (iTRAQ, SILAC, AQUA) or without labeling by Bottom-Up, Middle-Up/Middle-Down and Top-Down strategies
• Absolute protein quantification by targeted approaches (SRM, MRM, PRM)
• Analysis of intact proteins
• De novo protein sequencing by deploying different ion activation methods (ETC, ECD, IRMPD)
• Analysis of post-translational modifications (phosphorylation, glycosylation...) and access to ion mobility
• Enrichment in phospho or glycopeptides / proteins
• Identification of proteins by enzymatic digestion from various samples (powder, liquid, bone, tissue, plasma, gel...)
• Analysis of samples from cultural heritage, archaeology or paleontology
• Analysis of lipids such as triacylglycerols or metabolomics
• Mass spectrometry imaging (metabolites, lipids, peptides, proteins) from frozen or FFPE tissues
• Spatial proteomics (SpatiaXomics) by microextraction (LMJ LESA technology)
• Data processing and interpretation (protein arrays, GO terms)

Consulting services

We provide consulting services related to all our areas of expertise.


The MS4Omics platform is equipped with a range of latest generation instruments:
- for sample preparation (microspray system, piezoelectric microdeposition system, microextraction system, manual sublimation system, antigen unmasking system...),
- for proteomic and mass spectrometry analysis (high throughput MALDI-TOF imaging system, high spectral resolution MALDI system, ESI Q-Orbitrap Q-Exactive system...)
- for data analysis (Data server, Proteome Destroyer, computing station with Nvidia Tesla K20c GPU, FlexImaging 4, SCiLS Lab, data processing software...).


Nom Modèle Marque
MALDI-TOF RapiFlex/ Bruker
MALDI/ESI LTQ Orbitrap XL / Thermo Scientific
MS4Omics - MALDI-TOF/TOF Ultraflex II / 4800/ Bruler Daltonics / Absciex
ESI Q-Orbitrap Q-Exactive / Q-Exactive plus/ Thermo Scientific
Nano-UHPLC EASY-nLC 1000 / U3000 RSLC/ Thermo Scientific
FT-ICR SolariX XR 9.4 Tesla/ Bruker
MS4Omics - CZE ProteoLab PA 800/ Beckmann Coulter
Microspray system /
Piezoelectric microdeposit system CHIP1000 / Shimadzu
Microextraction system Triversa Nanomate LESA plus/ Advion


Affiliated institutions / organisations

CHU de Lille

Unit(s) of attachment


Regional strategic areas of activity

  • Health and Nutrition