• Chemistry and Materials

Miniaturisation for Synthesis, Analysis and Proteomics (MSAP)

Research unit - USR 3290


MSAP is a service and research unit that is at the interface of chemistry and biology. We are well recognized internationally for our instrumental and analytical developments related to mass spectrometry and we have built a solid reputation in the field of art analysis or identification of biological macromolecules. In chemistry, the originality of our unit is based on flow synthesis (from milli- to nano-fluidic) with developments in photochemistry or nanoparticle synthesis. MSAP also includes a mass spectrometry platform as well as a proteomics platform, and benefits from access to state-of-the-art equipment. We are open to all academic and industrial collaborations and propose services in line with our expertise.


  • Stéphanie Flament
  • Ahmed Mazzah


Bâtiment C4, Avenue Paul Langevin
Campus Cité Scientifique, Université de Lille


Miniaturisation for Synthesis, Analysis and Proteomics


Effectif total : 16

Personnel d'appui à la recherche : 4



• Mass spectrometry
• Proteomics
• Post-translational modifications (phosphorylation, glycosylation ...)
• Instrumental development
• Analytical developments: biomolecules, biobased
• Development of microreactors in organic synthesis
• Flow chemistry
• Chromatography
• Separation techniques
• Fine chemistry in multiscale microfluidic device
• Synthesis of nanoparticles
• Organic physico-chemistry
• Photochemistry

Example(s) of projects

• Actinometric analysis of compounds
• Multi-scale analysis of yeasts and their hydrolysates, from peptides to proteins
• Study of the works of Amadeo MODIGLIANI (LaM Lille Métropole Musée d'Art Moderne) 🡭

Example(s) of publications

• Colpaert, M., Kadouche, D., Ducatez, M., Pillonel, T., Kebbi-Beghdadi, C., Cenci, U., … & Colleoni, C. (2021). Conservation of the glycogen metabolism pathway underlines a pivotal function of storage polysaccharides in Chlamydiae. Communications biology, 4(1), 1-16. 🡭
• Venzac, B., Deng, S., Mahmoud, Z., Lenferink, A., Costa, A., Bray, F., … & Le Gac, S. (2021). PDMS Curing Inhibition on 3D-Printed Molds: Why? Also, How to Avoid It?. Analytical Chemistry. 🡭
• Le Guen, C., Mazzah, A., Penhoat, M., Melnyk, P., Rolando, C., & Chausset-Boissarie, L. (2021). Direct and Regioselective C (sp3)–H Bond Fluorination of 2-Alkylazaarenes with Selectfluor. Synthesis, 53(06), 1157-1162. 🡭
• Jóźwiak, P.; Ciesielski, P.; Zakrzewski, P.K.; Kozal, K.; Oracz, J.; Budryn, G.; Żyżelewicz, D.; Flament, S.; Vercoutter-Edouart, A.-S.; Bray, F.; Lefebvre, T.; Krześlak, A. Mitochondrial O-GlcNAc Transferase Interacts with and Modifies Many Proteins and Its Up-Regulation Affects Mitochondrial Function and Cellular Energy Homeostasis. Cancers 2021, 13, 2956. 🡭
• Mustapha Najib, Fabrice Bray, Simon Khelissa, Stephanie Flament, Elodie Richard, Khaled El Omari, Christian Rolando, Guillaume Delaplace, Monzer Hamze, Nour-Eddine Chihib. Effect of milk heat treatment on the molecular interactions during the process of Qishta-a Lebanese dairy product. International Dairy Journal 2021. 🡭

Discover the full list of publications here.

Collaborations/Partners/Scientific clients

University of Louvain (Belgium), University of Quebec (Canada), University of Gent (Belgium), University of Hong Kong (Hong Kong)

Collaborations/Partners/Private Clients

Roquette, Lesaffre, Mäder Group...

Services offers

Applications sectors

  • Health / wellness
  • Art / Culture
  • Education / Training
  • Science / Research

Services provided

• Identification of biological macromolecules
• Analysis of works of art
• Organic & bioorganic analysis of cultural heritage samples
• Proteomic, lipidomic, glycomic analysis
• Development of microfluidic devices
• Lipid analysis with identification of structures by fragmentation in CID or IRMPD
• Top-Down analyzes with nanochromatographic coupling
• Identification, quantification and analysis of proteins
• Analysis of post-translational modifications (phosphorylation, glycosylation ...)
• Enrichment in phospho or glycopeptides / proteins with the use of custom columns
• Analysis of lipids such as triacylglycerols or metabolomics

Consulting services

We provide consulting services related to all our areas of expertise.


We possess a wide range of equipment regrouped within the internal and external technological platforms comprising equipment for sample preparation, for proteomic analysis and by mass spectrometry, for data analysis. We also have:
• Mass spectrometers
• Nano-HPLC

See the detailed list of equipment: https://msap-lab.fr/instruments/


Affiliated institutions / organisations


Doctoral schools

Competitive cluster

Regional strategic areas of activity

  • Chemistry and Materials