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Health and Nutrition
Molecular mechanisms of N-glycosylation and associated pathologies
Research team
Our research focuses on the understanding of the molecular mechanisms of glycoyslation and the study of genetic and biochemical abnormalities in a group of rare genetic diseases called Congenital Disorders of Glycosylation (CDGs). The first part of our research activity is devoted to the identification and characterization of unresolved congenital disorders of glycosylation. This part is carried out in collaboration with the leaders of the European group on CDGs and exploits to the maximum the synergies and the interdisciplinary expertise that the consortium can provide. The second part is clearly fundamental and takes advantage of recently discovered genetic defects (new types of CDGs, new ER/Golgi deficiencies), which brings us fundamental knowledge on the molecular mechanisms controlling glycosylation pathways - an essential condition to develop therapies and cure patients.
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François Foulquier
Team manager
Bâtiment C9, Avenue Mendeleïev
Campus Cité Scientifique, Université de Lille
59655 VILLENEUVE D’ASCQ
http://ugsf-umr-glycobiologie.univ-lille1.fr/
Staff
Total staff : 9
Research staff : 7
Skills
• Generation and use of glycosylation-deficient cell models
• Creation of cellular assays to analyze glycosylation capacities (N- and O-)
• Generation of deficient and stable cell lines
• Expression of recombinant glycoproteins
• Study of intravesicular trafficking by confocal microscopy
• Glycosylation analysis by metabolic labeling and mass spectrometry
• Glycanic analysis of N-, O- and glycolipids
• Analysis of reticular glycosylation (NLO, fOS and LLO)
• Study of the glycosylation capacity by click chemistry and visualization by confocal microscopy (Use of fluorescent sugars)
• Quantification of Mn by an ICP-MS approach
• Creation of cellular assays to analyze glycosylation capacities (N- and O-)
• Generation of deficient and stable cell lines
• Expression of recombinant glycoproteins
• Study of intravesicular trafficking by confocal microscopy
• Glycosylation analysis by metabolic labeling and mass spectrometry
• Glycanic analysis of N-, O- and glycolipids
• Analysis of reticular glycosylation (NLO, fOS and LLO)
• Study of the glycosylation capacity by click chemistry and visualization by confocal microscopy (Use of fluorescent sugars)
• Quantification of Mn by an ICP-MS approach
Example(s) of projects
• Identification and characterization of numerous new human genetic defects leading to CDG glycosylation pathologies.
• Identification and functional characterization of a new Golgi proteins involved in glycosylation.
• Establishment of an efficient Manganese-based treatment for some CDG patients.
• Establishment of a methodology to visualize glycosylation deficiency by a chemistry-click approach in cells of CDG patients.
• Identification and functional characterization of a new Golgi proteins involved in glycosylation.
• Establishment of an efficient Manganese-based treatment for some CDG patients.
• Establishment of a methodology to visualize glycosylation deficiency by a chemistry-click approach in cells of CDG patients.
Example(s) of publications
• M. Houdou, E. Lebredonchel, A. Garat, S. Duvet, D. Legrand, V. Decool, A. Klein, M. Ouzzine, B. Gasnier, S. Potelle, F. Foulquier, Involvement of thapsigargin and cyclopiazonic acid–sensitive pumps in the rescue of TMEM165-associated glycosylation defects by Mn 2+, The FASEB Journal. (2018) fj.201800387R. doi:10.1096/fj.201800387R. 🡭
• Morelle, W., Potelle, S., Witters, P., Wong, S., Climer, L., Lupashin, V., Matthijs, G., Gadomski, T., Jaeken, J., Cassiman, D., Morava, E., and Foulquier, F. (2017) Galactose Supplementation in Patients With TMEM165-CDG Rescues the Glycosylation Defects. J. Clin. Endocrinol. Metab. 102, 1375–1386. 🡭
• Lebredonchel E, Houdou M, Hoffmann HH, Kondratska K, Krzewinski MA, Vicogne D, Rice CM, Klein A, Foulquier F. Investigating the functional link between TMEM165 and SPCA1. Biochem J. 2019 Nov 15;476(21):3281-3293. doi: 10.1042/BCJ20190488. PMID: 31652305. 🡭
• Blommaert E, Péanne R, Cherepanova NA, Rymen D, Staels F, Jaeken J, Race V, Keldermans L, Souche E, Corveleyn A, Sparkes R, Bhattacharya K, Devalck C, Schrijvers R, Foulquier F, Gilmore R, Matthijs G. Mutations in MAGT1 lead to a glycosylation disorder with a variable phenotype. Proc Natl Acad Sci U S A. 2019 May 14;116(20):9865-9870. doi: 10.1073/pnas.1817815116. Epub 2019 Apr 29. PMID: 31036665; PMCID: PMC6525510. 🡭
• Potelle S, Morelle W, Dulary E, Duvet S, Vicogne D, Spriet C, Krzewinski-Recchi MA, Morsomme P, Jaeken J, Matthijs G, De Bettignies G, Foulquier F. Glycosylation abnormalities in Gdt1p/TMEM165 deficient cells result from a defect in Golgi manganese homeostasis. Hum Mol Genet. 2016 Apr 15;25(8):1489-500. doi: 10.1093/hmg/ddw026. Epub 2016 Feb 1. PMID: 27008884. 🡭
• Morelle, W., Potelle, S., Witters, P., Wong, S., Climer, L., Lupashin, V., Matthijs, G., Gadomski, T., Jaeken, J., Cassiman, D., Morava, E., and Foulquier, F. (2017) Galactose Supplementation in Patients With TMEM165-CDG Rescues the Glycosylation Defects. J. Clin. Endocrinol. Metab. 102, 1375–1386. 🡭
• Lebredonchel E, Houdou M, Hoffmann HH, Kondratska K, Krzewinski MA, Vicogne D, Rice CM, Klein A, Foulquier F. Investigating the functional link between TMEM165 and SPCA1. Biochem J. 2019 Nov 15;476(21):3281-3293. doi: 10.1042/BCJ20190488. PMID: 31652305. 🡭
• Blommaert E, Péanne R, Cherepanova NA, Rymen D, Staels F, Jaeken J, Race V, Keldermans L, Souche E, Corveleyn A, Sparkes R, Bhattacharya K, Devalck C, Schrijvers R, Foulquier F, Gilmore R, Matthijs G. Mutations in MAGT1 lead to a glycosylation disorder with a variable phenotype. Proc Natl Acad Sci U S A. 2019 May 14;116(20):9865-9870. doi: 10.1073/pnas.1817815116. Epub 2019 Apr 29. PMID: 31036665; PMCID: PMC6525510. 🡭
• Potelle S, Morelle W, Dulary E, Duvet S, Vicogne D, Spriet C, Krzewinski-Recchi MA, Morsomme P, Jaeken J, Matthijs G, De Bettignies G, Foulquier F. Glycosylation abnormalities in Gdt1p/TMEM165 deficient cells result from a defect in Golgi manganese homeostasis. Hum Mol Genet. 2016 Apr 15;25(8):1489-500. doi: 10.1093/hmg/ddw026. Epub 2016 Feb 1. PMID: 27008884. 🡭
Collaborations/Partners/Scientific clients
National:
University of Rouen, University of Paris Descartes, University of Nancy, Imagine
International:
KULEUVEN, Institut De Duve, Charité, University of Arkansas, Yale University, LaJolla, Harvard University
University of Rouen, University of Paris Descartes, University of Nancy, Imagine
International:
KULEUVEN, Institut De Duve, Charité, University of Arkansas, Yale University, LaJolla, Harvard University
Applications sectors
- Health / wellness
- Education / Training
- Science / Research
Services provided
We are unable to offer any services at this time.
Consulting services
We provide consulting services related to all our areas of expertise (the analysis of glycosylation of glycoproteins).
• BSL2 cell culture rooms
• qPCR machines
• mass spectrometer
• biochip analyzer
• liquid chromatography systems
• particle size analyzer
• pipetting robot
• biacore T200; Biacore 3000
• all basic equipment (cell culture equipment, cell and molecular biology, ultracentrifuges, freeze-dryers, homogenizers, spectrophotometers, etc.).
To see the detailed list of equipment, refer to the profile of the STRUCTURAL AND FUNCTIONAL GLYCOBIOLOGY UNIT (UGSF) - UMR 8576 - https://www.pluginlabs-hautsdefrance.fr/en/entity/dca50749-5160-41c0-a814-eb8f6aea44ad/structural-and-functional-glycobiology-unit
Affiliated institutions / organisations
Unit(s) of attachment
Doctoral schools
Regional strategic areas of activity
- Health and Nutrition