• Health and Nutrition

Proteomics, Inflammatory Response, Mass Spectrometry (PRISM)

Research unit - U1192

Description

PRISM laboratory is a unique laboratory creating and developing new diagnostic tools, treatments, technologies and fundamental biological concepts, all oriented towards personalized medicine in oncology and neurology. We utilize state-of-the art molecular imaging and multi-OMICs technologies to better understand the pathophysiology of oncological and neurological disorders as well as to discover new treatment options. Our research activity is structured around two main axes: technological innovations and therapeutic innovations. We also developed two technological platforms: CLIC-Imaging, offering services in OMICS and mass spectrometry imaging; and 3D Cell OMICs, proposing services related to 3D cell culture (such as creation and maintenance of organoids and mixed spheroids, 3D organ bioprinting, exosome studies) for drug efficacy tests and for the development of new therapeutics. We are open to all academic and industrial collaborations and propose services in line with our expertise.

Contacts

  • Michel Salzet
    Scientific manager

Informations

Bâtiment SN3, Avenue Paul Langevin
Campus Cité Scientifique, Université de Lille
59655 VILLENEUVE D’ASCQ

https://laboratoire-prism.fr/

Proteomics, Inflammatory Response, Mass Spectrometry

Effectif

Effectif total : 72

Personnel d'appui à la recherche : 8

Expertises

Skills

Molecular imaging by mass spectrometry
SpiderMass in vivo mass spectrometry imaging
Proteomics
Lipidomics
Proteogenomics
Lipid imaging
Xenobiotic imaging
DMPK
ADME

Example(s) of projects

• SpiderMass : a novel instrument enabling non-targeted molecular information to be collected in-vivo for guided surgery. This system allows the minimally invasive collection of molecular data without requiring the use of probes or labeling.
• MacBeth : development of a therapeutic strategy allowing the reactivation of macrophages present in tumors.
• Ghost : development of mass spectrometry-based strategies to discover a new class of proteins “Alternative proteins” (altProts) or “Ghost Proteins”.

Example(s) of publications

• Cardon T, Franck J, Coyaud E, Laurent EMN, Damato M, Maffia M, Vergara D, Fournier I, Salzet M. Alternative proteins are functional regulators in cell reprogramming by PKA activation - Nucleic Acids Res. 2020 Aug 20;48(14):7864-7882. doi: 10.1093/nar/gkaa277 🡭
• Murgoci AN, Duhamel M, Raffo-Romero A, Mallah K, Aboulouard S, Lefebvre C, Kobeissy F, Fournier I, Zilkova M, Maderova D, Cizek M, Cizkova D, Salzet M. Location of neonatal microglia drives small extracellular vesicles content and biological functions in vitro - J Extracell Vesicles. 2020 Feb 18;9(1):1727637. doi: 10.1080/20013078.2020.1727637 🡭
• Seddiki K, Saudemont P, Precioso F, Ogrinc N, Wisztorski M, Salzet M, Fournier I, Droit A. Cumulative learning enables convolutional neural network representations for small mass spectrometry data classification - Nat Commun. 2020 Nov 5;11(1):5595 🡭
• Ogrinc N, Saudemont P, Balog J, Robin YM, Gimeno JP, Pascal Q, Tierny D, Takats Z, Salzet M, Fournier I. Water-assisted laser desorption/ionization mass spectrometry for minimally invasive in vivo and real-time surface analysis using SpiderMass - Nat Protoc. 2019 Nov;14(11):3162-3182. doi: 10.1038/s41596-019-0217-8 🡭
• Saudemont P, Quanico J, Robin YM, Baud A, Balog J, Fatou B, Tierny D, Pascal Q, Minier K, Pottier M, Focsa C, Ziskind M, Takats Z, Salzet M, Fournier I. Real-Time Molecular Diagnosis of Tumors Using Water-Assisted Laser Desorption/Ionization Mass Spectrometry Technology - Cancer Cell. 2018 Nov 12;34(5):840-851.e4 🡭

Collaborations/Partners/Scientific clients

National :

University of Lille

International :

Imperial College of London ( European Associated Laboratory - LANCET), Université Stanford (USA), University of California San Diego (USA), University of Florida (USA), University of Negev, Ben-Gurion (Israel), Center of Excellence in Neuroimmunology (Slovakia), Laval University (Canada), Univ. Salento (Italie)...

Collaborations/Partners/Private Clients

Oncovet Clinical Research (Labcomm O'DREAMS)

Services offers

Applications sectors

  • Health / wellness
  • Education / Training
  • Science / Research

Services provided

Molecular imaging by mass spectrometry (MALDI, DESI)
- SpiderMass in vivo mass spectrometry imaging
- Large scale non-targeted or targeted spatial proteomic analyzes
- Preparation of samples from cells, tissues, organoids, spheroids, body fluids, extracellular vesicles and exosomes.
- Identification of proteins or lipids
- Multi-omics analyzes
- Lipid imaging
- Xenobiotic imaging
- DMPK
- ADME
- Study of protein-protein interactions, post-translational modifications
- Data processing and statistical analysis

Consulting services

We provide consulting services related to all our areas of expertise.

Offres technologiques

  • Spidermass: guided surgery by real-time mass spectrometry
    SpiderMass is a device allowing the surgeons, in-real time during the surgery, to define the tumor margins, determine the existence of potential secondary tumor sites and get information on the potential aggressiveness of the tumor through tumor grading. Read more

Equipments

We posess state-of-the-art tools for sample preparation, proteomic and mass spectrometric analysis and data analysis:
- MALDI-TOF imaging system
- ESI-Q-Orbitrap mass spectrometer
- Xevo G2-XS-Qtof mass spectrometer
- EASY-nLC 1000 liquid chromatograph
- 3D bioprinter BIO X
- Sprayer HTX M5

See the detailed list of equipment: https://laboratoire-prism.fr/index.php/platforms/equipment

Biological materials

Breast cancer cell lines: MCF7, SKBR3
Ovarian cancer cell lines: SKOV3
Microglial cell lines: BV2

Ecosystem

Affiliated institutions / organisations

CHU de Lille
Inserm
University of Lille

Partner institution(s)

Centre Oscar Lambret

Doctoral schools

Regional strategic areas of activity

  • Health and Nutrition
    • Food ingredients
    • New therapeutic approaches

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Thematics

Current thematics

  • Health and Nutrition
    • Food ingredients
    • New therapeutic approaches
Title

Current content

Proteomics, Inflammatory Response, Mass Spectrometry
Type

Current content

Research unit
Description

Current content

PRISM laboratory is a unique laboratory creating and developing new diagnostic tools, treatments, technologies and fundamental biological concepts, all oriented towards personalized medicine in oncology and neurology. We utilize state-of-the art molecular imaging and multi-OMICs technologies to better understand the pathophysiology of oncological and neurological disorders as well as to discover new treatment options. Our research activity is structured around two main axes: technological innovations and therapeutic innovations. We also developed two technological platforms: CLIC-Imaging, offering services in OMICS and mass spectrometry imaging; and 3D Cell OMICs, proposing services related to 3D cell culture (such as creation and maintenance of organoids and mixed spheroids, 3D organ bioprinting, exosome studies) for drug efficacy tests and for the development of new therapeutics. We are open to all academic and industrial collaborations and propose services in line with our expertise.
Contact informations
Contact

Current content

Contact name Email phone number
SALZET Michel prism-u1192@univ-lille.fr (+33) 3 20 43 41 94
URL

Current content

https://laboratoire-prism.fr/

Address

Current content

Bâtiment SN3, Avenue Paul Langevin
Campus Cité Scientifique, Université de Lille
59655 VILLENEUVE D’ASCQ
Keywords

Current content

Mass spectrometry, Proteomics, OMICS, Oncoimmunology, Oncology, Neurology, Exoxome, Translational
Our search domains
Skills

Current content

Molecular imaging by mass spectrometry SpiderMass in vivo mass spectrometry imaging Proteomics Lipidomics Proteogenomics Lipid imaging Xenobiotic imaging DMPK ADME
Workforce

Current content

Nombre de chercheurs :
Nombre de doctorants :
Nombre de personnel d'appui à la recherche : 8
Effectif total : 72
Example(s) of projects

Current content

• SpiderMass : a novel instrument enabling non-targeted molecular information to be collected in-vivo for guided surgery. This system allows the minimally invasive collection of molecular data without requiring the use of probes or labeling. • MacBeth : development of a therapeutic strategy allowing the reactivation of macrophages present in tumors. • Ghost : development of mass spectrometry-based strategies to discover a new class of proteins “Alternative proteins” (altProts) or “Ghost Proteins”.
Example(s) of publications

Current content

• Cardon T, Franck J, Coyaud E, Laurent EMN, Damato M, Maffia M, Vergara D, Fournier I, Salzet M. Alternative proteins are functional regulators in cell reprogramming by PKA activation - Nucleic Acids Res. 2020 Aug 20;48(14):7864-7882. doi: 10.1093/nar/gkaa277 🡭 • Murgoci AN, Duhamel M, Raffo-Romero A, Mallah K, Aboulouard S, Lefebvre C, Kobeissy F, Fournier I, Zilkova M, Maderova D, Cizek M, Cizkova D, Salzet M. Location of neonatal microglia drives small extracellular vesicles content and biological functions in vitro - J Extracell Vesicles. 2020 Feb 18;9(1):1727637. doi: 10.1080/20013078.2020.1727637 🡭 • Seddiki K, Saudemont P, Precioso F, Ogrinc N, Wisztorski M, Salzet M, Fournier I, Droit A. Cumulative learning enables convolutional neural network representations for small mass spectrometry data classification - Nat Commun. 2020 Nov 5;11(1):5595 🡭 • Ogrinc N, Saudemont P, Balog J, Robin YM, Gimeno JP, Pascal Q, Tierny D, Takats Z, Salzet M, Fournier I. Water-assisted laser desorption/ionization mass spectrometry for minimally invasive in vivo and real-time surface analysis using SpiderMass - Nat Protoc. 2019 Nov;14(11):3162-3182. doi: 10.1038/s41596-019-0217-8 🡭 • Saudemont P, Quanico J, Robin YM, Baud A, Balog J, Fatou B, Tierny D, Pascal Q, Minier K, Pottier M, Focsa C, Ziskind M, Takats Z, Salzet M, Fournier I. Real-Time Molecular Diagnosis of Tumors Using Water-Assisted Laser Desorption/Ionization Mass Spectrometry Technology - Cancer Cell. 2018 Nov 12;34(5):840-851.e4 🡭
Collaborations/Partners/Scientific clients

Current content

National : University of Lille International : Imperial College of London ( European Associated Laboratory - LANCET), Université Stanford (USA), University of California San Diego (USA), University of Florida (USA), University of Negev, Ben-Gurion (Israel), Center of Excellence in Neuroimmunology (Slovakia), Laval University (Canada), Univ. Salento (Italie)...
Collaborations/Partners/Private Clients

Current content

Oncovet Clinical Research (Labcomm O'DREAMS)
Services offers
Training offers

Current content

Services offers

Current content

Molecular imaging by mass spectrometry (MALDI, DESI) - SpiderMass in vivo mass spectrometry imaging - Large scale non-targeted or targeted spatial proteomic analyzes - Preparation of samples from cells, tissues, organoids, spheroids, body fluids, extracellular vesicles and exosomes. - Identification of proteins or lipids - Multi-omics analyzes - Lipid imaging - Xenobiotic imaging - DMPK - ADME - Study of protein-protein interactions, post-translational modifications - Data processing and statistical analysis
Consulting services

Current content

We provide consulting services related to all our areas of expertise.
Equipments
Equipements spécifiques

Current content

  • MALDI TOF
    Rapiflex Tissue Typer/ Bruker
  • ESI-Q-Orbitrap
    Qexactive/ ThermoFisher
  • NanoUHPLC
    EASY-nLC 1000/ ThermoFisher
  • Microsprayer Preparation for MALDI Imaging
    Imageprep/ Bruker
  • Proteome Destroyer
    Proteome Destroyer with 64 Gb quad/ OmicsComputing
  • 3D bioprinter
    BIO X/ Cell Ink
  • Nanoparticles counter (exosomes)
    Nanosight ns 300/ Malvern
  • Q Tof
    Xevo G2-XS-Qtof/ Waters
  • Automaton Sprayer
    HTX M5/ HTX Imaging
  • Automatic collection and extraction system LCMS
    LESA nanomate triversa/ Advion
Ecosystem
Affiliated institutions / organisations

Current content

  • CHU de Lille
  • Inserm
  • University of Lille
Établissement(s) partenaire(s)

Current content

Centre Oscar Lambret
Unit(s) of attachment

Current content

Groups/Networks/Federations

Current content

Doctoral schools

Current content

Equipex/EquipEx+/ESR

Current content

Competitive cluster

Current content

Regional strategic areas of activity

Current content

  • Health and Nutrition
  • Food ingredients
  • New therapeutic approaches
Personal informations
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