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Digital World and Robotics
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Health and Nutrition
Lille Platforms in Biology and Health (PLBS)
Research unit - UMS 2014 - US 41
The seven platforms that make up PLBS provide access to the equipment and expertise needed to support research in biology and health. The technological, analytical and methodological approaches offered by PLBS range from the development of omics studies to cellular imaging, life imaging and functional exploration, as well as bioinformatics and biostatistics, high-throughput/high-content screening and experimental model housing facilities.
Primarily developed to meet the needs of academic research laboratories in life and health sciences at the University of Lille, PLBS is also open to national and international academic research activities as well as to industry.
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Philippe Boutin
Assistant director -
Sophie Crespin
Contact
1 place de Verdun
Faculté de Médecine - Pôle Recherche, Université de Lille
59045 LILLE
https://ums-plbs.univ-lille.fr/
Effectif
Effectif total : 84
Personnel de recherche : 1
Personnel d'appui à la recherche : 81
Skills
• HIGH THROUGHPUT AND HIGH CONTENT SCREENING PLATFORM (ARIADNE CRIBLAGE):
- High content and high throughput screening
- Screening automation
- Automated confocal microscopy
- Target characterization and modulator optimization
• BIOIMAGING CENTER LILLE (BICEL):
- Transmission electron imaging (TEM) and scanning (SEM)
- Photonic microscopy
- Conventional and spectral cytometry, flow imaging and cell sorting
- Sample preparation
- Intravital imaging
- Data analysis and interpretation
- Image analysis
• LILLE BIOINFORMATICS PLATFORM (BILILLE):
- Development of analysis pipelines and databases
- Integrative biology
- Gene, genome and protein annotation
- Phylogeny
- Systems biology
- Structural bioinformatics
- Development of bioinformatics and biostatistics tools
• GENOMICS PLATFORM (GO@L):
- "Whole genome" genomics
- Transcriptomics
- Metagenomics
- Whole genome sequencing and re-sequencing
- Re-sequencing of exomes of various qualities
- Targeted sequencing
- 16S targeted metagenomics
- RNA-seq and scRNA-seq
- DGE microarrays and DGE Nanostring
• LIFE IMAGING (LIIFE):
- In vivo imaging acquisition in MRI and PET
- Translational research
- Multidisciplinary approaches (radiology, nuclear medicine, engineering)
- Functional and behavioral exploration
- Image processing and analysis
• GLYCOMICS AND PROTEOMICS PLATFORM (PAGÉS/P3M):
- Composition and quantification of monosaccharides
- Study of complex mixture of glycans, poly- and oligosaccharides
- Structural glycobiology
- NMR spectroscopy (nuclear magnetic resonance)
- High throughput proteomics
- Study of post-translational modifications
- Glycoproteomics
- Native mass spectrometry
• ANIMAL FACILITIES:
- Rodent and aquatic model housing
- Breeding of transgenic lines
- Functional and metabolic explorations
- Body composition analysis
- High content and high throughput screening
- Screening automation
- Automated confocal microscopy
- Target characterization and modulator optimization
• BIOIMAGING CENTER LILLE (BICEL):
- Transmission electron imaging (TEM) and scanning (SEM)
- Photonic microscopy
- Conventional and spectral cytometry, flow imaging and cell sorting
- Sample preparation
- Intravital imaging
- Data analysis and interpretation
- Image analysis
• LILLE BIOINFORMATICS PLATFORM (BILILLE):
- Development of analysis pipelines and databases
- Integrative biology
- Gene, genome and protein annotation
- Phylogeny
- Systems biology
- Structural bioinformatics
- Development of bioinformatics and biostatistics tools
• GENOMICS PLATFORM (GO@L):
- "Whole genome" genomics
- Transcriptomics
- Metagenomics
- Whole genome sequencing and re-sequencing
- Re-sequencing of exomes of various qualities
- Targeted sequencing
- 16S targeted metagenomics
- RNA-seq and scRNA-seq
- DGE microarrays and DGE Nanostring
• LIFE IMAGING (LIIFE):
- In vivo imaging acquisition in MRI and PET
- Translational research
- Multidisciplinary approaches (radiology, nuclear medicine, engineering)
- Functional and behavioral exploration
- Image processing and analysis
• GLYCOMICS AND PROTEOMICS PLATFORM (PAGÉS/P3M):
- Composition and quantification of monosaccharides
- Study of complex mixture of glycans, poly- and oligosaccharides
- Structural glycobiology
- NMR spectroscopy (nuclear magnetic resonance)
- High throughput proteomics
- Study of post-translational modifications
- Glycoproteomics
- Native mass spectrometry
• ANIMAL FACILITIES:
- Rodent and aquatic model housing
- Breeding of transgenic lines
- Functional and metabolic explorations
- Body composition analysis
Example(s) of projects
Example(s) of publications
• High-Throughput Image-Based Aggresome Quantification (ARIADNE-Criblage) PMID: 32449635 🡭
• A microscopic phenotypic assay for the quantification of intracellular mycobacteria adapted for high-throughput/high-content screening (ARIADNE-Criblage) PMID: 24473237 🡭
• Détection d’évènements rares. La cytométrie en flux, 2ème édition (BICeL) 🡭
• MATAM: reconstruction of phylogenetic marker genes from short sequencing reads in metagenomes (bilille) PMID: 29040406 🡭
• MPAgenomics: an R package for multi-patient analysis of genomic markers (GO@L, bilille) PMID: 25495450 🡭
• MICRA: An automatic pipeline for fast characterization of microbial genomes from high-throughput sequencing data (GO@L) PMID: 29258574 🡭
• Prediction of Long-term Cognitive Functions after Minor Stroke, Using Functional Connectivity (LIIFE) PMID: 33402437 🡭
• Tenets, Methods, and Applications of Multifractal Analysis in Neurosciences (LIIFE) 🡭
• Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns (PAGés) PMID: 30405127 🡭
• REPRISAL: Mapping lignification dynamics using chemistry, data segmentation, and ratiometric analysis (TisBIO) PMID: 34687294 🡭
• A microscopic phenotypic assay for the quantification of intracellular mycobacteria adapted for high-throughput/high-content screening (ARIADNE-Criblage) PMID: 24473237 🡭
• Détection d’évènements rares. La cytométrie en flux, 2ème édition (BICeL) 🡭
• MATAM: reconstruction of phylogenetic marker genes from short sequencing reads in metagenomes (bilille) PMID: 29040406 🡭
• MPAgenomics: an R package for multi-patient analysis of genomic markers (GO@L, bilille) PMID: 25495450 🡭
• MICRA: An automatic pipeline for fast characterization of microbial genomes from high-throughput sequencing data (GO@L) PMID: 29258574 🡭
• Prediction of Long-term Cognitive Functions after Minor Stroke, Using Functional Connectivity (LIIFE) PMID: 33402437 🡭
• Tenets, Methods, and Applications of Multifractal Analysis in Neurosciences (LIIFE) 🡭
• Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns (PAGés) PMID: 30405127 🡭
• REPRISAL: Mapping lignification dynamics using chemistry, data segmentation, and ratiometric analysis (TisBIO) PMID: 34687294 🡭
Collaborations/Partners/Scientific clients
National:
Institute Pasteur Paris, Institute Necker Paris, Paris Orsay, University Paris Sud - Institute Galien, Paris Polytechnic Institute, Institute Curie, Institute Cochin, Technological University of Compiègne, University of Toulouse, Sorbonne University, University of Littoral Côte d'Opale, University of Rouen, University of Angers, Polytech Montpellier, University Hospital of Rouen, University Hospital of Nantes, University Hospital of Marseille, University Hospital of Amiens-Picardy, University Hospital of Bordeaux, Rothschild Foundation Hospital (Paris), UMR5240 - Microbiology, Adaptation and Pathogenesis, Platform "Exploration of Metabolism" INRA Clermont-Ferrand, U1086 ANTICIPE (Rouen), U1209 IAB (Grenoble), UMR7339 CRMBM (Marseille)
International :
Université Libre de Bruxelles (Belgium), Sciensano (Belgium), Weizmann Institute for Science (Israel), Paul Langherans Institute Dresden (Germany), University hospital of Munich (Germany), Nagoya University (Japan), University of Sheffield (Great Britain), Massachusetts General Hospital (USA), University of Chicago (USA), University hospital of Maastricht (Netherlands)
Institute Pasteur Paris, Institute Necker Paris, Paris Orsay, University Paris Sud - Institute Galien, Paris Polytechnic Institute, Institute Curie, Institute Cochin, Technological University of Compiègne, University of Toulouse, Sorbonne University, University of Littoral Côte d'Opale, University of Rouen, University of Angers, Polytech Montpellier, University Hospital of Rouen, University Hospital of Nantes, University Hospital of Marseille, University Hospital of Amiens-Picardy, University Hospital of Bordeaux, Rothschild Foundation Hospital (Paris), UMR5240 - Microbiology, Adaptation and Pathogenesis, Platform "Exploration of Metabolism" INRA Clermont-Ferrand, U1086 ANTICIPE (Rouen), U1209 IAB (Grenoble), UMR7339 CRMBM (Marseille)
International :
Université Libre de Bruxelles (Belgium), Sciensano (Belgium), Weizmann Institute for Science (Israel), Paul Langherans Institute Dresden (Germany), University hospital of Munich (Germany), Nagoya University (Japan), University of Sheffield (Great Britain), Massachusetts General Hospital (USA), University of Chicago (USA), University hospital of Maastricht (Netherlands)
Collaborations/Partners/Private Clients
Bioversys, Apteeus, GSK, Copalis, ANSES, OCR, Gènes Diffusion, Philips, General Electric, Siemens, Qynapse, Cleverdoc
Applications sectors
- Biotechnology
- Health / wellness
- Agri-food industry
- Education / Training
- Science / Research
Services provided
For specialized services, see the profiles of our platforms:
• GENOMICS PLATFORM (GO@L) 🡭
• GLYCOMICS AND PROTEOMICS PLATFORM (PAGÉS/P3M) 🡭
• LILLE BIOINFORMATICS PLATFORM (BILILLE) 🡭
• ANIMAL FACILITIES (EXPERIMENTAL RESOURCES)
• BIOIMAGING CENTER LILLE (BICEL) 🡭
• LIFE IMAGING (LIIFE)
• HIGH THROUGHPUT AND HIGH CONTENT SCREENING PLATFORM (ARIADNE CRIBLAGE) 🡭
• GENOMICS PLATFORM (GO@L) 🡭
• GLYCOMICS AND PROTEOMICS PLATFORM (PAGÉS/P3M) 🡭
• LILLE BIOINFORMATICS PLATFORM (BILILLE) 🡭
• ANIMAL FACILITIES (EXPERIMENTAL RESOURCES)
• BIOIMAGING CENTER LILLE (BICEL) 🡭
• LIFE IMAGING (LIIFE)
• HIGH THROUGHPUT AND HIGH CONTENT SCREENING PLATFORM (ARIADNE CRIBLAGE) 🡭
Training offers
The training programs proposed by PLBS include theoretical and practical training as well as workshops that are referenced in institutional training catalogs. Customized training programs can also be developed upon request.
• High content screening in confined environment LNSB2 and LNSB3
• Theory and practice of cytometry
• Theory and practice in photonic microscopy and videomicroscopy
• Practice in electron microscopy
• Training in image analysis with specialized software
• Theory and practical training in sample transparencies and 3D imaging
• Training in bioinformatics tools and approaches (databases, blast, annotation, alignment, phylogeny)
• Training in high-throughput sequencing data analysis (DNA sequencing, RNA-seq, Variants, Metagenomics, ChIP-seq)
• Training in data analysis with R and Bioconductor
• Training in glycotechnologies
• Theoretical and practical training in glycomics analysis
• NMR course applied to glycoconjugates
• Theoretical and practical training in functional and behavioral exploration in rodents
• High content screening in confined environment LNSB2 and LNSB3
• Theory and practice of cytometry
• Theory and practice in photonic microscopy and videomicroscopy
• Practice in electron microscopy
• Training in image analysis with specialized software
• Theory and practical training in sample transparencies and 3D imaging
• Training in bioinformatics tools and approaches (databases, blast, annotation, alignment, phylogeny)
• Training in high-throughput sequencing data analysis (DNA sequencing, RNA-seq, Variants, Metagenomics, ChIP-seq)
• Training in data analysis with R and Bioconductor
• Training in glycotechnologies
• Theoretical and practical training in glycomics analysis
• NMR course applied to glycoconjugates
• Theoretical and practical training in functional and behavioral exploration in rodents
Consulting services
We provide consulting services related to all our areas of expertise.
Specialized equipment that requires expert knowledge, or that is located in specific and/or particularly sensitive areas, can be accessed in collaborative mode or via a research or service agreements.
To see the detailed list of equipment, refer to the profiles of our platforms:
• GENOMICS PLATFORM (GO@L) 🡭
• GLYCOMICS AND PROTEOMICS PLATFORM (PAGÉS/P3M) 🡭
• LILLE BIOINFORMATICS PLATFORM (BILILLE) 🡭
• ANIMAL FACILITIES (EXPERIMENTAL RESOURCES)
• BIOIMAGING CENTER LILLE (BICEL) 🡭
• LIFE IMAGING (LIIFE)
• HIGH THROUGHPUT AND HIGH CONTENT SCREENING PLATFORM (ARIADNE CRIBLAGE) 🡭
Biological materials
• Chemical libraries
• siRNA library
• Murine, zebrafish, xenopus models
• siRNA library
• Murine, zebrafish, xenopus models
Affiliated institutions / organisations
Partner institution(s)
Groups/Networks/Federations
Equipex/EquipEx+/ESR
Doctoral schools
Competitive cluster
Regional strategic areas of activity
- Digital World and Robotics
- Artificial intelligence, image processing, data science
- Engineering, software design, open source software
- Health and Nutrition
- Food ingredients
- Medicine of the future: new health equipment and e-health
- New therapeutic approaches
- Safe, healthy and sustainable food